Functional single-cell assays with droplet flow cytometry
Join this FACSDiscover™ User Group presentation at the Horizon Next Global Tour: Advancing Cell Analysis, Revealing Biology, where we showcase how imaging-enabled flow cytometry and droplet technology are redefining functional single-cell analysis.
In this session, our Field Application Scientist presents a novel workflow combining Samplix Xdrop with the BD FACSDiscover S8, leveraging CellView imaging to capture both population-level data and high-resolution views of individual droplets.
This approach allows:
- Visualization of double-emulsion droplets
- Detection of cell encapsulation within droplets
- Direct observation of cell–cell interactions, such as NK cell-mediated killing of target cells
- Measurement of functional activity, including Granzyme B substrate signal within single droplets
By compartmentalizing cells in droplets, signals remain isolated, avoiding the mixing effects seen in bulk assays. This opens new possibilities to:
- Study immune cell function at true single-cell resolution
- Monitor cytotoxicity, cytokine capture, or antibody secretion in confined microenvironments
- Strengthen workflows for cell and gene therapy development, where functional validation is critical
This presentation highlights how droplet flow cytometry can bridge the gap between what cells are and what they do, providing a powerful new lens on complex biological systems.
