ATMP Sweden ATMP Sweden Finalized initiatives CAMP CAMP Projects GMP pluripotent derivatives

GMP pluripotent derivatives

GMP production of pluripotent stem cells and its derivatives (continuation)

The overall goal is to establish pluripotent stem cells (iPS and/or hES cells)
for derivation of GMP approved cells for clinical studies.

Time period: 11/2021 – 11/2023

Lead: Anders Lindahl, Gothenburg University

Involved partners: University of Gothenburg, Karolinska Institutet, Umeå University, Region Västerbotten, Vecura/Karolinska Universitetssjukhuset, Takara Bio Europe, VERIGRAFT 

Pluripotent stem cells are the technical foundation for new cell-based therapies as well as research tools for academia and the pharmacological industry. Derivation of iPS cell-lines of GMP grade have been be done in collaboration with partners and is complying with the
regulations as determined by the Swedish MPA (Läkemedelsverket). Robust differentiation protocols have been developed (chondrocytes, Schwann cells). The project part of a lager EU, VR, Vinnova-funded projects.

Work plan:

  • Establish standardized iPS culture system for all cell- lines
  • Perform derivation experiments performed including pellet cultures
  • RNA sequencing of monolayer culture and Bioinformatics
  • Derivation of Schwann cell protocol
  • Single cell sequencing of human chondrocytes
  • Decellularization of nerves from rats
  • Single cell sequencing of induced chondrocytes from iPS cells

Publications

”Karolinska Institutet Human Embryonic Stem Cell Bank.”